2,812 research outputs found
Integrated Laboratory Demonstrations of Multi-Object Adaptive Optics on a Simulated 10-Meter Telescope at Visible Wavelengths
One important frontier for astronomical adaptive optics (AO) involves methods
such as Multi-Object AO and Multi-Conjugate AO that have the potential to give
a significantly larger field of view than conventional AO techniques. A second
key emphasis over the next decade will be to push astronomical AO to visible
wavelengths. We have conducted the first laboratory simulations of wide-field,
laser guide star adaptive optics at visible wavelengths on a 10-meter-class
telescope. These experiments, utilizing the UCO/Lick Observatory's Multi-Object
/ Laser Tomography Adaptive Optics (MOAO/LTAO) testbed, demonstrate new
techniques in wavefront sensing and control that are crucial to future on-sky
MOAO systems. We (1) test and confirm the feasibility of highly accurate
atmospheric tomography with laser guide stars, (2) demonstrate key innovations
allowing open-loop operation of Shack-Hartmann wavefront sensors (with errors
of ~30 nm) as will be needed for MOAO, and (3) build a complete error budget
model describing system performance. The AO system maintains a performance of
32.4% Strehl on-axis, with 24.5% and 22.6% at 10" and 15", respectively, at a
science wavelength of 710 nm (R-band) over the equivalent of 0.8 seconds of
simulation. The MOAO-corrected field of view is ~25 times larger in area than
that limited by anisoplanatism at R-band. Our error budget is composed of terms
verified through independent, empirical experiments. Error terms arising from
calibration inaccuracies and optical drift are comparable in magnitude to
traditional terms like fitting error and tomographic error. This makes a strong
case for implementing additional calibration facilities in future AO systems,
including accelerometers on powered optics, 3D turbulators, telescope and LGS
simulators, and external calibration ports for deformable mirrors.Comment: 29 pages, 11 figures, submitted to PAS
Rigorous Determination of the Stoichiometry of Protein Phosphorylation Using Mass Spectrometry
Quantification of the stoichiometry of phosphorylation is usually achieved using a mixture of phosphatase treatment and differential isotopic labeling. Here, we introduce a new approach to the concomitant determination of absolute protein concentration and the stoichiometry of phosphorylation at predefined sites. The method exploits QconCAT to quantify levels of phosphorylated and nonphosphorylated peptide sequences in a phosphoprotein. The nonphosphorylated sequence is used to determine the absolute protein quantity and serves as a reference to calculate the extent of phosphorylation at the second peptide. Thus, the stoichiometry of phosphorylation and the absolute protein concentration can be determined accurately in a single experiment
Synchronous dynamics of zooplankton competitors prevail in temperate lake ecosystems
Although competing species are expected to exhibit compensatory dynamics (negative temporal covariation), empirical work has demonstrated that competitive communities often exhibit synchronous dynamics (positive temporal covariation). This has led to the suggestion that environmental forcing dominates species dynamics; however, synchronous and compensatory dynamics may appear at different length scales and/or at different times, making it challenging to identify their relative importance. We compiled 58 long-term datasets of zooplankton abundance in north-temperate and sub-tropical lakes and used wavelet analysis to quantify general patterns in the times and scales at which synchronous/compensatory dynamics dominated zooplankton communities in different regions and across the entire dataset. Synchronous dynamics were far more prevalent at all scales and times and were ubiquitous at the annual scale. Although we found compensatory dynamics in approximately 14% of all combinations of time period/scale/lake, there were no consistent scales or time periods during which compensatory dynamics were apparent across different regions. Our results suggest that the processes driving compensatory dynamics may be local in their extent, while those generating synchronous dynamics operate at much larger scales. This highlights an important gap in our understanding of the interaction between environmental and biotic forces that structure communities
"No Going Back?" The final report of the Effective Records Management Project
The overall objective of the Effective Records Management project was the provision of āprotocols and tools for the effective management of information in the digital order, with particular attention to information held in a document-based formā . The project developed a demonstrator system which addressed the full range of issues involved in the management of a ātestbedā of digital records in the form of documents, from their creation through distribution and use to their final disposal or permanent retention. The testbed selected was the records of a subset of the universityās committees and was used to develop ideas and to assess how well they worked in practice. This project report indicates that the ERM project provides an investment in the future, opportunities for better information use or re-use, identification of legal risks and identification of good practice whether you be an administrator, a computing scientist, an archivist, a librarian or an information services manager
"No Going Back?" The final report of the Effective Records Management Project
The overall objective of the Effective Records Management project was the provision of āprotocols and tools for the effective management of information in the digital order, with particular attention to information held in a document-based formā . The project developed a demonstrator system which addressed the full range of issues involved in the management of a ātestbedā of digital records in the form of documents, from their creation through distribution and use to their final disposal or permanent retention. The testbed selected was the records of a subset of the universityās committees and was used to develop ideas and to assess how well they worked in practice. This project report indicates that the ERM project provides an investment in the future, opportunities for better information use or re-use, identification of legal risks and identification of good practice whether you be an administrator, a computing scientist, an archivist, a librarian or an information services manager
Pump Pulse Bandwidth-Activated Nonlinear Phononic Coupling in CdWO
To control structure-function relationships in solids with light, we must
harness the shape of the potential energy surface, as expressed in anharmonic
coupling coefficients. We use two-dimensional terahertz (THz) spectroscopy to
identify trilinear coupling between sets of vibrational modes in CdWO. It
is generally understood that efficient trilinear coupling occurs when the
frequencies of two coupled modes add or subtract to the frequency of the third
mode. Interestingly, we observe that this condition is not necessary: the THz
driving-pulse itself can activate the coupling by contributing broad frequency
content to the initial motion of the excited modes. Understanding that the
bandwidth of the driving force can activate energy-flow pathways has broad
implications for coherent control of collective modes using intense THz light
pulses.Comment: 27 Pages, 15 Figure
Increased expression of the chemokines CXCL1 and MIP-1Ī± by resident brain cells precedes neutrophil infiltration in the brain following prolonged soman-induced status epilepticus in rats
<p>Abstract</p> <p>Background</p> <p>Exposure to the nerve agent soman (GD) causes neuronal cell death and impaired behavioral function dependent on the induction of status epilepticus (SE). Little is known about the maturation of this pathological process, though neuroinflammation and infiltration of neutrophils are prominent features. The purpose of this study is to quantify the regional and temporal progression of early chemotactic signals, describe the cellular expression of these factors and the relationship between expression and neutrophil infiltration in damaged brain using a rat GD seizure model.</p> <p>Methods</p> <p>Protein levels of 4 chemokines responsible for neutrophil infiltration and activation were quantified up to 72 hours in multiple brain regions (i.e. piriform cortex, hippocampus and thalamus) following SE onset using multiplex bead immunoassays. Chemokines with significantly increased protein levels were localized to resident brain cells (i.e. neurons, astrocytes, microglia and endothelial cells). Lastly, neutrophil infiltration into these brain regions was quantified and correlated to the expression of these chemokines.</p> <p>Results</p> <p>We observed significant concentration increases for CXCL1 and MIP-1Ī± after seizure onset. CXCL1 expression originated from neurons and endothelial cells while MIP-1Ī± was expressed by neurons and microglia. Lastly, the expression of these chemokines directly preceded and positively correlated with significant neutrophil infiltration in the brain. These data suggest that following GD-induced SE, a strong chemotactic response originating from various brain cells, recruits circulating neutrophils to the injured brain.</p> <p>Conclusions</p> <p>A strong induction of neutrophil attractant chemokines occurs following GD-induced SE resulting in neutrophil influx into injured brain tissues. This process may play a key role in the progressive secondary brain pathology observed in this model though further study is warranted.</p
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